Rhizobia transform legume nodules via native T-DNA transfer systems

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1. Proche Même méthode, autre problème Pas pertinent

   A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crop species

   Freddie J. King, Juan Carlos Lopez-Agudelo, Christopher Stephens et al. · 2026 · PLoS Biology · Pas encore résumé

   AS109, une souche de Rhizobium rhizogenes, permet l’expression transitoire dans diverses plantes, tandis que l’article étudie le transfert natif d’ADN par des rhizobiums dans des nodules.

   Résumé scientifique

   Agrobacterium-mediated transient expression has revolutionised plant research, enabling numerous landmark discoveries across diverse areas of plant biology. Yet this powerful approach remains largely confined to solanaceous species, leaving most economically important crop families without a comparable rapid assay platform. Here, we show that an engineered Rhizobium rhizogenes strain, AS109, mediates efficient transient expression across diverse dicot species spanning multiple taxonomic families, consistently outperforming commonly used laboratory agrobacterial strains. Leveraging the broad host range of AS109, we establish a suite of functional assays in faba bean (Vicia faba), including pr…

2. Proche Contexte plus large Pas pertinent

   Transcriptional silencing of a conjugative Sinorhizobium megaplasmid upon transfer to different rhizobial species

   Cristina Robles de la Cruz, L. Cervantes, G. Torres Tejerizo et al. · 2026 · Frontiers in Microbiology · Pas encore résumé

   Cette étude suit le transfert d’un mégaplasmide conjugatif entre rhizobiums et Agrobacterium, plutôt que le transfert d’ADN vers des cellules végétales.

   Résumé scientifique

   Horizontal gene transfer of large conjugative megaplasmids drives bacterial evolution, however, it forces new hosts to rapidly adapt to a massive influx of exogenous DNA; even the acquisition of small plasmids can have variable effects on host genome activity and fitness. In Sinorhizobium fredii GR64 (symbiont of the common bean) the megaplasmid pSfr64a (191 kb) displays a high conjugative transfer rate. Interestingly, when pSfr64a is transferred to Rhizobium etli (another bean symbiont) conjugative transfer from this host became nearly undetectable, whereas in Agrobacterium tumefaciens it is maintained at a high rate. To unravel the host-plasmid dynamics driving this host-specific response,…

3. Proche Autre approche du problème Pas pertinent

   DipTRANS: an improved method for in planta transformation and genome engineering in Nicotiana benthamiana.

   Fa-Zhan Qi, Wei-Wei Chen, Jon P. Cody et al. · 2026 · Trends in Biotechnology · Pas encore résumé

   DipTRANS utilise Agrobacterium pour transformer Nicotiana benthamiana de façon héréditaire, tandis que l’article examine le transfert natif de T-DNA par des rhizobiums.

   Résumé scientifique

   Plant transformation remains constrained by labor-intensive tissue culture. Our previous work showed that direct delivery of developmental regulators (DRs) can induce de novo meristems on plants, offering a promising transformation approach. In this resource article, we introduced DipTRANS (Direct in planta Transformation), an optimized, soil-based heritable transformation platform for Nicotiana benthamiana that bypasses sterile culture entirely. DipTRANS is built on DR-induced de novo meristem formation. After optimizing parameters, including regulator combinations, Agrobacterium strain, and infiltration density, DipTRANS yielded transformation efficiencies to 46.7%. Developmental abnormali…

4. Proche Contexte plus large Pas pertinent

   Type III effectors of symbiotic Rhizobia include diverse predicted transcriptional and post-transcriptional modulators

   Albin Teulet, S. Schornack · 2026 · PLoS Biology · Pas encore résumé

   Cette étude prédit les fonctions d’effecteurs rhizobiens sécrétés par un système de type III, et non le transfert de T-DNA dans les nodules.

   Résumé scientifique

   Rhizobia are soil bacteria that establish nitrogen-fixing symbioses with legumes. While many rhizobia use a Type III Secretion System to deliver “Nodulation Outer Protein” (Nop) effectors, some uniquely use these proteins to initiate nodule organogenesis, bypassing classical signalling. The molecular functions of these effectors remain largely unknown due to extreme sequence divergence. Using AlphaFold2-mediated structural proteomics, we identified a modular architecture in rhizobial effectors composed of 22 distinct structural units. We reveal that many Nop effectors are cryptic transcriptional or post-transcriptional regulators, harbouring unrecognised nucleic acid–binding modules and RNA-…

5. Proche Contexte plus large Pas pertinent

   Rhizobial type III effectors as regulators of root-nodule symbiosis in legumes.

   Alicia Camuel, Albin Teulet, P. del Cerro · 2026 · Journal of Experimental Botany · Pas encore résumé

   Cette synthèse décrit comment les effecteurs de type III régulent la symbiose des nodules, un mécanisme distinct du transfert de T-DNA étudié dans l’article.

   Résumé scientifique

   The rhizobia-legume nitrogen-fixing symbiosis begins when plants perceive rhizobial Nod factors (NF), activating the Common Symbiosis Signalling Pathway (CSSP) that drives nodule organogenesis. Rhizobia also inject Type III effectors (T3E) into host cells through a Type III Secretion System (T3SS) related to those of pathogenic bacteria. These effectors were long viewed only as suppressors of plant immunity and determinants of host compatibility, but this picture has changed. Recent studies revealed that rhizobial effectors (known as Nodulation Outer Proteins, Nops) such as NopT, NopM, NopL, NopP and NopZ directly target NF receptors or associated kinases, thereby regulating CSSP activation.…

6. Proche Contexte plus large Pas pertinent

   Genetic code expansion enables plant-directed control of bacterial activity.

   Vivian Zhong, Michaela A. Jones, Avaniek Cabales et al. · 2026 · Nature Biotechnology · Pas encore résumé

   Cette étude programme l’activité de Bacillus subtilis associé aux plantes par expansion du code génétique, sans examiner le transfert d’ADN bactérien aux plantes.

   Résumé scientifique

   Microorganisms have the potential to deliver growth-promoting benefits to plants and crops; however, to be useful in a field setting, their gene expression should be programmable and restricted to specific plant hosts and conditions. Here, using O-methyl-L-tyrosine (OMY) as a model compound, we establish noncanonical amino acids (ncAAs) as a tool for plant-based control of associated microbial activity. We use genetic code expansion to engineer OMY-dependent control of protein synthesis in the soil bacterium Bacillus subtilis. Engineered B. subtilis strains express an orthogonal aminoacyl-tRNA synthetase and tRNA pair that, upon provision of OMY, results in suppression of in-frame amber stop…

7. Proche Autre approche du problème Pas pertinent

   Establishment of an efficient Agrobacterium rhizogenes-mediated hairy root transformation system for functional analysis in tomato

   Si-Yi Zhang, Long-Zhu Huang, Jun-Jie Liu et al. · 2026 · Frontiers in Plant Science · Pas encore résumé

   Cette étude optimise la transformation de racines de tomate par Agrobacterium rhizogenes, tandis que l’article teste le transfert natif d’ADN par des rhizobiums.

   Résumé scientifique

   Tomato is an important model for functional genomics in Solanaceae, and elucidating its gene functions provides valuable insights into conserved genetic regulatory networks across dicotyledonous plants. Hairy root transformation is a key technology for functional studies in tomato. However, systematic optimization of its parameters remains limited. Here, we established an efficient Agrobacterium rhizogenes-mediated hairy root transformation system for tomato cv. Micro-Tom. Using the RUBY visual reporter gene, we systematically evaluated multiple parameters, including A. rhizogenes strain, bacterial optical density (OD600), inoculation duration, acetosyringone (AS) concentration, and the type…

8. Proche Même méthode, autre problème Pas pertinent

   Suppression of plant immune responses enhances Agrobacterium-mediated transient expression in Nicotiana benthamiana.

   Yun-Jee Kang, Fen Xie, Ge Bai et al. · 2026 · Plant Science · Pas encore résumé

   Cette étude utilise Agrobacterium tumefaciens pour exprimer transitoirement des gènes dans Nicotiana benthamiana, comme approche de transformation végétale différente du transfert rhizobien natif.

   Résumé scientifique

   Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana is widely used for recombinant protein production and gene-function studies. However, agroinfiltration can activate host stress and defense responses, thereby limiting heterologous protein accumulation. To distinguish Agrobacterium-associated transcriptional responses from effects of the infiltration procedure, we performed RNA sequencing (RNA-seq) using an infection group (GV3101 carrying empty p1300), a buffer-only mock-infiltration group (Mock), and untreated wild-type (WT) plants. Relative to the mock group, the infection group contained 3,529 upregulated and 2,487 downregulated differentially expressed gene…