Exécution de notre outil de synthèse sur un nouvel article
Vérifiez l’article ci-dessous, puis lancez sa synthèse.
A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crop species
Freddie J. King (Imperial College London), Juan Carlos Lopez‐Agudelo (National Chung Hsing University, Institute of Plant and Microbial Biology, Academia Sinica), Christopher Stephens (University of Cambridge), May Htet Aung (Institute of Plant and Microbial Biology, Academia Sinica, Academia Sinica), Tarhan Ibrahim (Imperial College London), Enoch Lok Him Yuen (Imperial College London), Ao Chen (Imperial College London), Nick Moritz Eilmann (Imperial College London), Saskia Jenkins (Imperial College London), Cristina Vuolo (Imperial College London), Yueh-Ning Swee (Institute of Plant and Microbial Biology, Academia Sinica, Academia Sinica), Wei-Jia Liu (Institute of Plant and Microbial Biology, Academia Sinica, Academia Sinica), Samuel Bruty (John Innes Centre, University of Cambridge), AmirAli Toghani (University of East Anglia), Chih‐Horng Kuo (National Chung Hsing University, Institute of Plant and Microbial Biology, Academia Sinica), Erh‐Min Lai (National Chung Hsing University, Institute of Plant and Microbial Biology, Academia Sinica), Jiorgos Kourelis (Imperial College London), Lida Derevnina (University of Cambridge), Chih-Hang Wu (National Chung Hsing University, Institute of Plant and Microbial Biology, Academia Sinica), Tolga O. Bozkurt (Imperial College London)
Résumé : Agrobacterium-mediated transient expression has revolutionised plant research, enabling numerous landmark discoveries across diverse areas of plant biology. Yet this powerful approach remains largely confined to solanaceous species, leaving most economically important crop families without a comparable rapid assay platform. Here, we show that an engineered Rhizobium rhizogenes strain, AS109, mediates efficient transient expression across diverse dicot species spanning multiple taxonomic families, consistently outperforming commonly used laboratory agrobacterial strains. Leveraging the broad host range of AS109, we establish a suite of functional assays in faba bean (Vicia faba), including protein localisation, RNA interference-mediated gene silencing, cell-surface elicitor recognition screens, nucleotide-binding leucine-rich repeat receptor (NLR) activation, and infection cell biology at the host-pathogen interface. We further demonstrate that both singleton NLRs and sensor-helper NLR pairs from Solanaceae retain effector recognition and cell death activity when transferred into faba bean, establishing a rapid platform for evaluating cross-family transferability of disease-resistance genes. AS109 thus provides an accessible and versatile chassis for functional genomics in non-model crops, bridging the widening gap between hypothesis generation and experimental validation across diverse plant species.