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Genetic code expansion enables plant-directed control of bacterial activity
Vivian Zhong (Stanford University), Michaela A. Jones (University of Delaware), Avaniek Cabales (University of Delaware), Alice Gevorgyan (Stanford University), René Inckemann (Stanford University), A. L. Johnson (Stanford University), Sumudu S Karunadasa (Carnegie Institution for Science, Carnegie Department of Plant Biology), Amanda M. Forti (University of Delaware), Shou‐Ling Xu (Carnegie Institution for Science, Carnegie Department of Plant Biology), Aditya M. Kunjapur (University of Delaware), Jennifer A. N. Brophy (Howard Hughes Medical Institute, Stanford University)
Résumé : Microorganisms have the potential to deliver growth-promoting benefits to plants and crops; however, to be useful in a field setting, their gene expression should be programmable and restricted to specific plant hosts and conditions. Here, using O-methyl-L-tyrosine (OMY) as a model compound, we establish noncanonical amino acids (ncAAs) as a tool for plant-based control of associated microbial activity. We use genetic code expansion to engineer OMY-dependent control of protein synthesis in the soil bacterium Bacillus subtilis. Engineered B. subtilis strains express an orthogonal aminoacyl-tRNA synthetase and tRNA pair that, upon provision of OMY, results in suppression of in-frame amber stop codons in target transcripts. We engineer Arabidopsis, tomato and poplar to biosynthesize OMY, showing that plant-derived OMY can stimulate gene expression in both model and wild soil bacteria. This demonstrates that inducible and tissue-specific expression of a single biosynthetic enzyme by the plant enables on-demand control over microbial activity, establishing ncAAs as a tool for programming plant-microorganism partnerships.