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Low Cost LAMP and RT-LAMP v1
Tamara Matúte, Isaac Núñez, Fernán Federici
Abstract: This protocol collection specifies how to carry out LAMP or RT-LAMP (reverse transcription - Loop-mediated isothermal amplification) reactions with homemade reagents and home-brewed enzymes. It describes how to prepare all the solutions and buffers to perform the reactions. This collection was developed in the context of COVID-19 pandemic to provide access to RT-LAMP tools in accordance with the principles of ReClone community initiative. It is the result of information exchange and reagent sharing with a huge community of collaborators from ReClone, gLAMP and JOGL.