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Résumé : Protocol for photostimulation, to be combined with any other electrophysiology/imaging recording approach. Once the desired patch clamp recording (e.g., cell attached, whole cell, perforated patch) and/or imaging (e.g., genetically encoded sensor or synthetic dye) configuration is achieved and a stable recording is established, photostimulation can be used to stimulate presynaptic terminals/cell expressing an opsin of interest (e.g., Chronos or Channelrhodopsin) or to release a photo-sensitive caged compound (e.g., RUBI-GABA, RUBI-Glutamate, MNI-glutamate...), and test the effect on the experimental outcome of interest (e.g., excitatory/inhibitory response).