# Optimization of Production Protocol of Alkaline Protease by Streptomyces pulvereceus

**Authors:** D. Jayasree, T. D. Sandhya Kumari, P. B. Kavi Kishor, M. Vijaya Lakshmi, M. Lakshmi Narasu  
**arXiv:** [1002.0048](https://arxiv.org/abs/1002.0048)  
**Date:** 2010-01-30  
**Summary page:** https://www.summarizepaper.com/en/paper/1002.0048v1/

## Key points

- Study focuses on optimization of production protocol of alkaline protease by Streptomyces pulvereceus MTCC 8374
- Maximum level of enzyme production occurs during early stationary phase in submerged fermentation
- Optimum pH for enzyme production is 9.0
- Optimal inoculum and temperature are 3% and 33°C respectively
- 0.3% starch results in highest protease production among carbon sources, followed by maltose, xylose, and fructose
- 1.0% casein shows highest yield of protease production among nitrogen sources, followed by soybean meal, yeast extract, and malt extract
- Concentration of 1% NaCl results in maximum level of protease production during optimization experiments with varying concentrations of NaCl
- Streptomyces pulvereceus MTCC 8374 has high enzymatic activity under optimized conditions

## Summary

The study focuses on the optimization of the production protocol of alkaline protease by Streptomyces pulvereceus MTCC 8374, an extracellular alkaline protease-producing species isolated from soil. During submerged fermentation, it was found that the maximum level of enzyme production occurred during the early stationary phase. The optimum pH for enzyme production was determined to be 9.0, while the optimal inoculum and temperature were 3 percent and 33°C respectively. In terms of carbon sources, 0.3 percent starch resulted in the highest protease production followed by maltose, xylose and fructose. Among different nitrogen sources tested, 1.0 percent casein showed the highest yield of protease production followed by soybean meal, yeast extract and malt extract. Further optimization experiments with varying concentrations of NaCl (0.5 percent, 1.0 percent and 1.5 percent) revealed that a concentration of 1 percent NaCl resulted in the maximum level of protease production. Overall, this protease profile demonstrates Streptomyces pulvereceus MTCC 8374's potential as a valuable source for industrial applications due to its high enzymatic activity under optimized conditions.