# Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution

**Authors:** James H. Rice  
**arXiv:** [0708.3336](https://arxiv.org/abs/0708.3336)  
**Date:** 2007-08-24  
**Summary page:** https://www.summarizepaper.com/en/paper/0708.3336v1/

## Key points

- Fluorescence microscopy is widely used in imaging biological systems
- The resolution of images obtained is limited by the laws of diffraction
- Researchers have been developing methods to image beyond the diffraction limit using far-field fluorescence microscopy techniques
- Rapid progress has been made in this area, with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit
- James H. Rice's review article titled "Far-field Fluorescence Microscopy Beyond The Diffraction Limit: Fluorescence Imaging With Ultrahigh Resolution" outlines these developments and their application to biology
- The article provides an overview of various far-field fluorescence methods that enable ultrahigh resolution imaging
- The article discusses future trends and directions in this field
- Rice's review offers a comprehensive analysis of super-resolution fluorescence microscopy techniques that can help researchers better understand biological systems at a higher level of detail than previously possible

## Summary

The use of fluorescence microscopy in imaging biological systems is widespread and has been used to gain insights into the inner workings of these systems. However, the resolution of images obtained is limited by the laws of diffraction. To address this limitation, researchers have been developing methods to image beyond the diffraction limit using far-field fluorescence microscopy techniques. In recent years, rapid progress has been made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. James H. Rice's review article titled "Far-field Fluorescence Microscopy Beyond The Diffraction Limit: Fluorescence Imaging With Ultrahigh Resolution" outlines these developments and their application to biology. The article provides an overview of various far-field fluorescence methods that enable ultrahigh resolution imaging and discusses future trends and directions in this field. With 27 pages, 2 figures and 2 tables, Rice's review offers a comprehensive analysis of super-resolution fluorescence microscopy techniques that can help researchers better understand biological systems at a higher level of detail than previously possible.